Theme of the School:
Behavioural Animal Models for Neurochemical Research
Venue: National University of Singapore, Department of Pharmacology, Yong Loo Lin School of Medicine, Singapore
Date: To be held between 14th to 18th of July 2020.
Goals of the School:
In spite of the various advances in in vitro and computer modelling methods that allow for the replacement of animals, characterising the physical presentation and behaviour of an animal is integral to understanding the underlying bases of the disease being modelled or neurochemical agents being tested. Hence, being able to effectively phenotype an animal model is an important tool for researchers. This proposed 4.5-day school draws expertise from various institutions in Singapore, such as the National University of Singapore (NUS), Yale-NUS, Duke-NUS and Agency for Science, Technology and Research (A*STAR), with the aim to expand the participants’ understanding of various aspects related to using animal models for basic biomedical research, and enable them to employ these techniques in their future research. Participants will be introduced to a simple, invertebrate model, the drosophila, which can be easily deployed with minimal infrastructure, as well as increasingly complex animal models that require more infrastructure, such as the zebrafish and mouse.
The school syllabus will cover:
Instructional methods will be student-centred and interactive. Classroom sessions will involve interactive discussions. To facilitate progressive mastery of learning, core knowledge concepts will be covered first in classroom sessions and then reinforced through experience in demonstrations and hands-on sessions or workshops. Schedule: Day 1 (14th July, Tues, at NUS) The school begins with an introduction to various animal models that are commonly employed in behavioural phenotyping research. This day will mostly focus on the use of the mouse model, with a lecture on the setting up of a mouse colony and related ethical issues. There will also be a hands-on opportunity for the participants to learn how to handle mice, proper restraint and substance administration techniques. During the lunch break, participants are to attend a concurrent poster presentation session, where they will get to share their research projects with other participants and the attending faculties.
The school begins with an introduction to various animal models that are commonly employed in behavioural phenotyping research. This day will mostly focus on the use of the mouse model, with a lecture on the setting up of a mouse colony and related ethical issues. There will also be a hands-on opportunity for the participants to learn how to handle mice, proper restraint and substance administration techniques. During the lunch break, participants are to attend a concurrent poster presentation session, where they will get to share their research projects with other participants and the attending faculties.
|09:00 - 09:30||Administrative Matters||Registration|
|09:30 - 10:00||Welcome address (Gavin Dawe, Pharmacology, NUS)||Classroom|
|10:00 - 11:00||An introduction to various animal models & use of rodent models in neuroscience (Gavin Dawe, Pharmacology, NUS)||Classroom|
|11:00 - 12:00||Rodent module: Breeding and colony management, maintenance of transgenic lines, ethics involved (Lois Zitzow, Comparative Medicine, NUS)||Classroom|
|12:00 - 14:00||Welcome Lunch/Poster session by participants||Scientific interaction|
|14:00 - 17:00||Rodent module: Introductory training for mouse handling (Comparative Medicine, NUS)||Demonstration & Hands-on|
Day 2 sees a continuation of the mouse model, with a focus on behavioural phenotyping methodology. The morning session will set the stage for the mouse behavioural phenotyping observation sessions on Day 4. In addition, students will learn about how to design behavioural experiment and handle the data generated effectively. In the afternoon, they will be introduced to the zebrafish model. The day ends with a visit to the Comparative Medicine animal facility at NUS.
|09:00 - 10:00||Rodent module: Methodology, advantages, disadvantages, & use of genetically modified mice (Mahmoud Pouladi, A*Star)||Classroom|
|10:00 - 11:00||Statistics module: Good experimental design & calculation of power to determine sample size (Seyed Ehsan Saffari, Duke-NUS)||Classroom|
|11:00 - 12:00||Statistics module: Use of statistics in behavioural studies (Seyed Ehsan Saffari, Duke-NUS)||Workshop|
|12:00 - 14:00||Lunch|
|14:00 - 15:00||Statistics module: Moving beyond P values: Everyday data analysis with estimation plots (Adam Claridge-Chang, Duke-NUS, A*STAR)||Classroom|
|15:00 - 16:00||Fish module: Breeding and colony managements, health (Shannon Heo, Comparative Medicine, NUS)||Classroom|
|16:00 - 17:00||Rodent/Fish module: Visit to Comparative Medicine animal facility (Comparative Medicine, NUS)||Facility visit|
Participants will travel to A*STAR facilities to learn about the drosophila and zebrafish models. After the classroom activities, they will be divided into 4 groups, (6-7 participants per group), for the demonstration and hands-on session. There will be 4 stations for the students to rotate through:
|09:00 - 10:00||Drosophila module: Methodology, advantages and disadvantages of using Drosophila (Sherry Aw, A*Star)||Classroom|
|10:00 - 11:00||Fish module: Methodology, advantages and disadvantages of using Zebrafishes (Ajay Mathuru, Yale-NUS, A*Star)||Classroom|
|11:00 - 12:00||Drosophila/Fish module: Drosophila and Zebrafish behaviour hands-on and observation session.||Demonstration & Hands-on|
|12:00 - 14:00||Lunch|
|14:00 - 17:00||Drosophila/Fish module: Drosophila and Zebrafish behaviour hands-on and observation session.||Demonstration & Hands-on|
The participants will be introduced to an open source behavioural analysis software. After which, they will get two mouse behavioural testing observation sessions. The participants will be divided into 4 groups that rotate through 4 stations. Most of the behavioural tests featured do not require complex equipment, with the testing arena being something that can be made in a local workshop.
Session 1: Motor behavioural tests – the balance beam, open field, rotarod and gait analysis.
Session 2: Affective behavioural tests – the plus maze, zero maze, light/dark box, and tail suspension test. Students will learn and practise how to score in real-time.
The participants will consolidate their learning from the previous days. They will give a 5-minute slide presentation on how they may apply what they have learnt to their own research. Faculties will be present to facilitate discussions. The school will close with a farewell lunch and a city tour.
|09:00 - 10:00||Keynote address: Kim Jee Hyun (Florey Institute of Neuroscience and Mental Health, Melbourne, Australia)||Classroom|
|12:30 - 13:00||Closing Remarks (Gavin Dawe)|
|13:00 - 15:00||Farewell Lunch (speakers + personnel involved)||Social|
|15:00 - 17:00||Singapore City Tour (Mahmoud Pouladi, Judy Sng)||Social|
Contact us for more information.
Yong Loo Lin School of Medicine
National University of Singapore
16 Medical Drive, Singapore 117600
Tel: +65 6516 3264